Stable Cell Line Development   1. Notice - The stable cell lines generated are for research use only (for clinical use or any other purpose, you should obtain a license from Genenmed Inc.). - Genenmed provides a high-performance vector system for expressing intra-cellularly or extra-cellularly (secreted to the culture supernatant).   2. General information   1) About expression vector system - The CMV promoter (including an intron downstream) drives expression of the target gene. - A puromycin-resistant gene is expressed on a separate vector - Stable clones are generated by co-transfection and selected using Puromycin. - Customer-designed expression vector can also be used.   2) For customers - Codon optimization service is available. - Customer should provide host cell line. - If host cell line needs special culture conditions, extra-charge is requested.   3.Services - Pooled stable cells - Clonally selected high-producer cells   4. Questions - TEL : 82-2-3474-5800 - E-mail : sales@genenmed.com

1. pAb1HX expression vector 1. You can send us your own sequence already validated    (please see the matched restriction sites in pAb1HX vector for cloning) 2. You can send us the sequence information. Then, we will synthesize codon-optimized DNA and clone it to the vector 3. The cloned pAb1HX vector is linearized and then co-transfected with the linearized pPURO vector. 2. pAb2HX expression vector 1. You can send us your own sequence already validated     (please see the matched restriction sites in pAb2HX vector for cloning) 2. You can send us the sequence information. Then, we will synthesize codon-optimized DNAs and clone them to the vector 3. The cloned pAb2HX vector is linearized and then co-transfected with the linearized pPURO vector.