AnyGeneKI^TM , a CRISPR/Cas9-based Gene Knock-in

1. Notice

- AnyGeneKI^TM, a gene knock-in (KI) service, utilizes CRISPR/Cas9 system

- The knock-incell lines generated are for research use only (for clinical use or any other purpose,

you should obtain a license from Genenmed Inc.)

2. General information

1) About gene Knock_In

- Gene knock-in can be used for gene knock-out, deletion, insertion, etc

- Gene knock-in is accomplished by two Target Guide RNA/Cas9vectors and a knock-in donor vector

(three plasmids or three adenoviruses)

- Unlike the plasmid based knock-in, the adenovirus-based knock-in does not leave

(or extremely low probability) an exogenous DNA in the established cell line

2) For customers

- Customer should provide host cell line

- If host cell line needs special culture conditions, extra-charge is requested

- Customer should provide the target sequence (20 nucleotide in length) for gene knock-in

(see Figure for detail)

- Customer should provide the antibody for Western blotting

3.Services

- Plasmid-based knock-in

- Adenovirus-based knock-in

4. Questions

- TEL: 82-2-3474-5800

- E-mail: sales@genenmed.com