1. Procedure of Cell Freezing
1) Remove cell culture medium.
2) For adherent cells, wash and trypsinize cells. For suspend cells, start with Step 4.
3) Resuspend cells with complete culture medium.
4) Centrifuge to collect cells.
5) Remove culture medium as much as possible.
6) Resuspend cells with CellFreezer™ at 107~108 cells/ml
7) Incubate in ice for 10 minutes.
8) Add DMSO (final 10%(v/v), Cell Culture Grade) and mix gently.
9) Dispense cells (1ml) into the cryovials and store at deep freezer.
2. Instructions for Thawing Frozen Cells
1) Thaw frozen cells in 37℃ water bath.
2) Add cells directly to 10ml complete culture medium.
3) Incubate cells under appropriate culture condition for 24 hours.
4) Change plating medium with fresh culture medium.
3. Cell Viability Test
